Human skin explants can be used for a variety of purposes: to determine the anti-inflammatory activity against known TLR ligands, to understand inflammation associated with injection site reactions, dendritic cell activation assessment and as adjuvant assessment for vaccine delivery.
Aquila has experience in human skin culture and can tailor projects specifically to suit your needs. Healthy human skin samples are ethically sourced from patients undergoing corrective abdominal surgery. A mid-high number of biopsies per patient sample can be achieved to allow for a moderate throughput of compound screening.
Explants are cultured for up to 7 days, per protocol requirements, and supernatant analysed for cytokine content by ELISA or bead array as appropriate, to determine inflammatory or anti-inflammatory activity.