Mixed lymphocyte reaction (MLR) assays can be used to understand the effects of biologics or small molecules on T cell activation, an important immune response in the tumour microenvironment (TME).

There are different MLR protocols available:

  • “one-way” or “two-way” MLR systems using PBMCs
  • Autologous MLR assay using monocyte-derived dendritic cells (Mo-DC) and CD4+ T cells from the same donor, and anti CD3/CD28 as the stimulator
  • Allogenic MLR assay using Mo-DC and CD4+ T cells from HLA-mismatched donors (immature or mature)

We can advise on the most suitable MLR to understand your drug candidate mechanism of action (MOA), and tailor the assay accordingly.

We offer both custom and standard assay readouts, such as cytokine expression (e.g. IFN-γ), T cell activation (e.g. CD80), proliferation (e.g. CFSE) and cell cycle (e.g. Ki-67) measured by ELISA and/or flow cytometry.

Contact us today to learn more about our human MLRs.