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Multiplex IF/IHC

We offer multiplex immunohistochemistry (IHC) and immunofluorescence (IF) assays to enable detection of multiple targets on one section. Our automated approach uses Leica robots to give you a consistent, reproducible and high-throughput service that can be adapted for multiple protocols and detection strategies.

Key advantages of using IF multiplex, include:

  • Identifying multiple targets in the same section
  • Up to six fluorescent endpoints + counterstain (7-plex)
  • Sensitive tyramides used for IF
  • Multiple antibodies from the same species
  • Conservation of precious tissue samples
  • Target validation and distribution data

We partner with vendors, including OracleBio, to bring our clients a complete specialist histology service. We can quantify multiple fluorescent markers present in the same section, enabling you to evaluate the spatial relationships between immune cell types and your target.

By combining multiplex IF and RNAscope® in the same sample, we can also help validate your target antibodies using RNA expression.

Aquila also offers custom multiplex biomarker panels using a large range of validated and optimised antibodies, and we continue to develop the list of targets we can detect.

If you would like Aquila to validate or optimise your target, or to discuss your project further, please get in touch.